评论
打印
PCR confirmation was performed on every sample
聚合酶链式反应PCR被用于每个样本
The study site was at 13 villages under jurisdiction of 4 health establishments
研究位置包括13个村分属4个健康设施辖区
And these are located in one of the main focus of transmission in the Peruvian Amazon, It is here, okay
这些都位于秘鲁亚马逊地区主要传播地，这里
We use 2 techniques for genotyping
我们使用了两种基因型分析技术
The first one is PCR-RFLP technique which means that the primary product obtain it from PCR amplification here
第一种是PCR-RFLP技术，最初产品通过PCR扩增获得
where then digest it using 2 restriction enzymes and then we got to profiles
然后两种限制性内切酶进行消化得到DNA片段
The second type of technique was, we use 9 different variable-number tandem repeat markers located in that area
第二种技术我们使用了该区域9种不同的可变数目串联重复序列标记
including this certain merozoite surface protein in which we now is under selective pressure
包括这个裂殖子表面蛋白，我们知道它处在选择压力下
So by comparing additional type of these loci together at baseline
通过对比这些基因位点在基线
and at the recurrent sample relapses can be distinguished reliably from re-infections
及在再发作中的情况，复发能够可靠地同再感染区分开来
For that analysis, we consider relapse if both were identical
分析中如果两者相同，则认为是复发
and re-infection if at least for one of the markers we use it was different
如果其中一个标记不同，则认为是再感染
